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    • Deglycosylation ProtocolCitationsApplicationsUser GuideGlycan Mass SpectrometryDeglycosylation Protocol PNGase F Deglycosylation Protocol Denaturing deglycosylation of 100 µg of glycoproteins and analysis Add 100 µg of glycoprotein and fill with UltraPure H2O (milliQ, NanoPure, etc) until 70 µl. Add 10 µl of Denaturing Buffer (5% SDS, 400 mM DTT). Incubate at 100°C for 10 minutes. Add 10 µl of [...]