Transferases

9

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    • N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase     Size: 4 mU

      Introduction: B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose,  Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and  N-Acetyllactosamine (LacNAc) Type 1 O-glycans.

      B3Gal Transferase can be used for synthesis of  the precursor of H type 1 antigen of ABO histo-blood group. The H type 1 antigen in itself is the precursor of  the following type 1 antigens:  antigens A, antigen B, Lewis a (Le a ), Lewis b (Le b ) and  Sialyl Lewis a (sLe a).

      Source: E. coli cells with a cloned bacterial B3GalT gene.

      Storage Buffer: The enzyme is provided in solution containing Tris buffer, NaCl and 10% glycerol

      Activity: The activity per µL is 40 µUnits/µL or 248 pmol/min/μL

      Milliunit Definition: One milliunit will transfer 1.0 nanomole of galactose from UDP-galactose to 4-Nitrophenyl N-acetyl-β-D-glucosaminide per min at pH 7.5 at 37 °C.

      Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.

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