Glycan Profiling and Identification

We offer UPLC-FLD/MS of 2-AB, 2-AA or procainamide labeled glycans profiling service of cell, tissue or glycoprotein samples, and MALDI-TOF MS based glycan structure identification, including α-2,3/ α-2,6 differential sialylation

Sample preparation includes :

  • Glycoprotein fraction isolation
  • Glycan release and labeling
  • Chromatographic separation and glycan profiling.

Additional services:

  • MALDI-TOF MS differential sialylation analysis
  • MS/MS fragmentation for glycan structures confirmation or unknown structures identification
  • Glycosidase treatment
                                                                                                                                   Request a quote

 

IgG type Antibodies Quantitative Glycan Analysis

Our glycan quantification service is based on the isotopic dilution method and applied to the Absolute quantification of N-glycans in IgG type monoclonal antibodies by MALDI-TOF MS, both in low and high-throughput (up to 96 samples).

 Application to:

  • Glycoanalysis of biologics
  • Absolute quantification of biosimilar glycosylation

 

 

Request a quote

Why our service?

– Only service that currently provides Absolute Glycan Quantification (pmol).

– Get your results in less than than 48 h after sample arrival.

– Glycan quantification with internal standards (not based on quantification of attached fluorophores).

Simple sample preparation and data acquisition.

Service

Report includes:

– Absolute Glycan Quantification (pmol).

– Relative Glycan Quantification (%).

– Absolute Monosaccharide Quantification (pmol).

– Relative Monosaccharide Quantification (%).

– Total Glycan amount (pmol).

Sample requirements


-Minimum required sample amount is 50 μg of human IgG type antibodies expressed in CHO cell lines.

– optimal sample amount 50-500 μg of IgG type antibody expressed in CHO cell lines.

-For optimal sample condition ship at 4 ºC or – 20 ºC.

Advertisements