Comprehensive glycan analysis for precise characterization

Advanced structural and purity analysis of custom and biological glycans using LC-MS, MALDI-TOF, NMR and specialized techniques — supporting compound validation and glycan profiling across treatments or biological conditions

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What we offer in glycoanalysis & glycan profiling

Reliable validation for complex glycan and glycopeptide structures

Browse our analytical services, from glycan profiling to advanced structural characterization.

Glycan analysis services

Glycan profiling

N-glycan profiling

N-glycan profiling by LC-FLD MS
N-glycan profiling by MALDI TOF
N-glycopeptide profiling (by LC-FLD MS, by MALDI TOF, by HPAE-PAD)

O-glycan profiling

O-glycan profiling by LC-FLD-MS
O-glycan profiling by MALDI-TOF MS and LC-MS

Glycolipid profiling

Glycolipid Profiling by MALDI-TOF MS and LC-MS

High throughput (HTP) profiling

Total serum N-glycan
Single protein N-glycan profiling studies after immunoprecipitation

Special immunoglobulins (IgG)

Serum IgG-N-glycan profiling by LC-MS (biomarker studies)
Serum IgG N-glycan Profiling by MALDI-TOF MS + Absolute Quantification by CarboQuant Technology
Recombinant therapeutic IgG Fc-glycan profiling by MALDI-TOF MS and CarboQuant Technology
Fc fragment analysis by LC-MS

Glycosylation structural analysis

General structural assignment

Overall glycosidic linkage determination: exo-glycosidase treatment and re-analysis by LC-MS
Sialic acid linkage and NeuAc/NeuGc ratios determination
MS/MS fragment analysis or Via GU values
Glycan identification with pure glycan standards

Monosaccharide Composition Determination

Analysis of N and O-glycans.

Polysaccharide analysis

Advanced NMR methods for sequence analysis, branching patterns, and repeating units determination
Monosaccharide composition by GC-MS
Relative monosaccharide quantification by LC-MS (fluorescence/UV labeling)
Unlabeled oligosaccharide separation by HPAE-PAD

Glycoprotein analysis – Functional and structural determination

Intact Mass by MALDI-TOF

Lectin Glycoblotting

N-glycosylation Site Occupation Analysis

Glycosylation Site-specific glycan profile of purified proteins by LC-MS

Microarray technology

Lectin microarrays

Lectin binding profile of a wide range of biological samples (purified protein, serum samples, exosomes, glycoconjugates)
Custom-made protein and lectin arrays (c-type lectins, siglecs)

Glycan microarrays

150-glycan array to test carbohydrate binding preferences
Custom-made glycan array from personalized libraries and custom-made glycan libraries synthesized in Asparia

Trusted by leaders in biotech, academia and diagnostics

More than 350 clients across the globe already trust Asparia for their glycoscience needs — from top universities to biotech innovators and diagnostic developers

Applications of our glycan solutions

We support scientists working in therapeutic development, diagnostics, and beyond — across a wide range of research areas.

Oncology

Tumor-associated carbohydrate antigens (TACA), glycan biomarkers, targeted delivery (GalNAc).

Infectious diseases

Vaccine development, pathogen recognition, and antiviral compounds.

Immune and inflammatory diseases

Monitoring disease progression, therapeutic development.

Microbiome

Host-microbe interactions, metabolite analysis, and polysaccharides.

Biomarkers & diagnostics

Early detection and personalized medicine approaches.

Other fields

Food science, veterinary applications, HMOs, cosmetics and more.

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CarboQuant standards. Our patented technology

CarboQuant standards are patented synthetic ¹³C-labeled N-glycans, fully developed in-house and used as internal standards for absolute glycan quantification. They are available individually and integrated into our workflows for glycan analysis

Designed for consistency, reproducibility and precision, CarboQuant expands the possibilities of quantitative glycomics — including challenging applications and analysis.

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Custom Synthesis Lab at Asparia Glycomics, helping with your reasearch and development.

Research & Scientific Publications

The expertise behind our analytical services is grounded in over a decade of scientific research

At Asparia, we actively participate in R&D projects and contribute to international publications. Our technologies — including CarboQuant — are the result of this ongoing commitment to innovation.

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Frequently asked questions about custom glycan synthesis

What types of samples do you accept for glycan analysis?

We offer custom glycan analysis services for a wide range of biological samples, including:
– Serum antibodies and/or full serum glycan profiles (immunoglobulins) (10-20 μL).
– Saliva and urinary glycoproteins.
– Recombinant purified proteins (50-100 μg).
– Protein extracts.
– Exosomes.
– Plant proteins.
– Animal tissues (20 mg).
– Cell lines and cell line membranes (3×10^6).
– We also offer custom method development for non-standard or unpurified samples.

What techniques do you use for glycan analysis?

We apply a combination of LC-MS, MALDI-TOF, NMR, and other specialized techniques depending on your sample type and goals, ensuring structural accuracy, quantification, and reproducibility.

Which equipment do you use for glycan analysis?

The company has access to:
– High-end chromatography and mass spectrometry systems, including a Bruker Ultraflex Extreme MALDI-TOF/TOF for rapid analysis of glycans using matrix-assisted desorption/ionization (MALDI).
– Ultra-high-performance chromatography (UHPLC) separations of glycomics and glycoproteomics samples using hydrophobic (reversed phase) or hydrophilic interaction (HILIC) stationary phases.
The UHPLC systems are coupled with a fluorimeter and high-resolution Q-TOF mass spectrometers. This includes the latest generation of Waters Synapt XS QTOF.

Can you analyze both N-glycans and O-glycans?

Yes. We provide dedicated workflows for both N-glycan and O-glycan analysis, including structural elucidation, quantification, and sialic acid profiling when required.

Do you offer glycan quantification or just profiling?

We offer both. Our services include relative and absolute quantification of glycans using internal standards such as our proprietary CarboQuant technology.

Do you provide glycosylation site mapping?

Yes. We perform glycosylation site mapping to identify and characterize glycosylation patterns on proteins, supporting both research and biopharmaceutical development.

Can you develop a custom analytical method for my project?

Absolutely. If your sample or application requires a tailored approach, we offer custom method development to meet your specific scientific or regulatory needs.