N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase Size: 4 mU
Introduction: B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose, Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and N-Acetyllactosamine (LacNAc) Type 1 O-glycans.
B3Gal Transferase can be used for synthesis of the precursor of H type 1 antigen of ABO histo-blood group. The H type 1 antigen in itself is the precursor of the following type 1 antigens: antigens A, antigen B, Lewis a (Le a ), Lewis b (Le b ) and Sialyl Lewis a (sLe a).
Source: E. coli cells with a cloned bacterial B3GalT gene.
Storage Buffer: The enzyme is provided in solution containing Tris buffer, NaCl and 10% glycerol
Activity: The activity per µL is 40 µUnits/µL or 248 pmol/min/μL
Milliunit Definition: One milliunit will transfer 1.0 nanomole of galactose from UDP-galactose to 4-Nitrophenyl N-acetyl-β-D-glucosaminide per min at pH 7.5 at 37 °C.
Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.