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Beta-(1,3)-N-Acetylgalactosaminyltransferase Size: 10 mU
Introduction:
B3GlcNAcT is a β-1,3-N-acetylgalactosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans.
It can function also as a B3GlcNAcT (Beta-(1,3)-N-Acetylglucosamilyltransferase)
Activity:
Overnight incubation with 4 µL of this enzyme will produce 1 mg of product. If higher amount of product is needed the enzyme will continuously add GlcNAc for at least one week.
Reaction conditions are: Donor: UDP-GLcNAc, substrate: pNP-Lactose, product: pNP-Lactose-GlcNAc, reaction temperature: 23 ºC , incubation time: 18 hours.
The specific activity is 975.7 pmol/min/μg
Source: E. coli cells with a cloned bacterial B3GlcNAc Transferase gene.
Storage Buffer: The enzyme is provided as lyophilized powder solution containing Tris buffer and NaCl
Milliunit Definition: One milliunit will transfer 1.0 nanomole of GlcNAc (N-Acetyl-D-glucosamine) from UDP-GlcNAc (Uridine diphosphate N-acetylglucosamine) to Galβ1→4Glcβ-O-pNP (4-Nitrophenyl β -D-lactopyranoside) per min at pH 7.5 at 25 °C.
Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.
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B3GlcNAcT is a β-1,3-N-acetylglucosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans.
