PNGase F_500 mU INVOICE 2019-39

SKU: PNGaseF-500mU Lyophilized Protein for R&D

PNGase F_500 mU     INVOICE 2019-39

Introduction: CarboClip is a recombinant peptide-N-Glycosidase F (PNGase F), from Elizabethkingia sp, overexpressed in E. coli that contains a hexahistidine tag in its C-term and cleaves between the GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. CarboClip is provided glycerol-free as a lyophilized powder containing Tris-HCl, NaCl2, and Na2EDTA.

Activity: After reconstitution in 750 μL of UltraPure H2O, 1 μl of CarboClip can deglycosylate > 95% of ribonuclease B (1 mg) in less than 30 minutes. Activity is calculated on a 12 % SDS-PAGE and percent of deglycosylation using densitometry.

Storage: Lyophilized powder: -80 °C and -20 °C >1 year. At room temperature 10 days. Reconstituted protein should be stored at 4 °C and is stable for 2-3 months.