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Lectin Microarray Our lectin array system provides a high throughput platform for rapid characterization of glycosylation in proteins, antibodies, hormones, and other glycoconjugates. The technology utilizes a panel of up 48 different lectins that specifically bind to different N and O- glycans epitopes including :
Blood groups B and H
Lewis X antigen
Complex branched glycans
The panel includes 6 replicates of each immobilized lectin along with immobilized control samples (printing buffer containing BSA) Method Summary Analyses are performed in triplicate. The glycoproteins samples are labeled with a cy-3/cy-5 dyes, incubated with the lectin array and the interactions quantified by fluorescence detection. Data is provided as histograms showing the corresponding lectin interaction profiles with each sample. Raw data (xlsx format) and a fluorescence image of the incubated lectin microarrays is also provided Pricing Prices are based in the number of chips used.
Introduction: CarboClip is a recombinant peptide-N-Glycosidase F (PNGase F), from Elizabethkingia sp, overexpressed in E. coli that contains a hexahistidine tag in its C-term and cleaves between the GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. CarboClip is provided glycerol-free as a lyophilized powder containing Tris-HCl, NaCl2, and Na2EDTA.
Activity: After reconstitution in 750 μL of UltraPure H2O, 1 μl of CarboClip can deglycosylate > 95% of ribonuclease B (1 mg) in less than 30 minutes. Activity is calculated on a 12 % SDS-PAGE and percent of deglycosylation using densitometry.
Storage: Lyophilized powder: -80 °C and -20 °C >1 year. At room temperature 10 days. Reconstituted protein should be stored at 4 °C and is stable for 2-3 months.