The MALDI-TOF based quantification method is compatible with a high-troughput performance, yielding a complex data set which can be treated easily with the mAbsolute Quantification Software.
- Data formats: The software was developed for Bruker data formats, and also accepts data in common formats as .csv, analyzing tenths or hundreds of experiments automatically.
- Quality control: The software evaluates the quality of the quantification data by calibrating the different glycan isotopologues
- Results report: The software generates a customized report regarding absolute and relative quantification data.
Isotopic Dilution Method based absolute quantification
The mAbsolute quantification software takes advantage of the N-glycan isotopologue profiles, with 4-5 abundant isotopologues in the MS spectra of known natural abundances. This characteristic makes that CarboQuant standards are present in the sample at different known concentrations which can be used for absolute quantification.
The software uses up to four isotopologues of each light and heavy glycan to perform a robust absolute quantification.
The mAbsolute Quantification Software can be also adapted to any other biomolecule or metabolite and used to perform MS-based absolute quantification studies.
If you have any question or are interested in a given application, please contact with us.