Products
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Beta-(1,3)-N-Acetylgalactosaminyltransferase Size: 10 mU
Introduction:
B3GlcNAcT is a β-1,3-N-acetylgalactosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans.
It can function also as a B3GlcNAcT (Beta-(1,3)-N-Acetylglucosamilyltransferase)
Activity:
Overnight incubation with 4 µL of this enzyme will produce 1 mg of product. If higher amount of product is needed the enzyme will continuously add GlcNAc for at least one week.
Reaction conditions are: Donor: UDP-GLcNAc, substrate: pNP-Lactose, product: pNP-Lactose-GlcNAc, reaction temperature: 23 ºC , incubation time: 18 hours.The specific activity is 975.7 pmol/min/μg
Source: E. coli cells with a cloned bacterial B3GlcNAc Transferase gene.
Storage Buffer: The enzyme is provided as lyophilized powder solution containing Tris buffer and NaCl
Milliunit Definition: One milliunit will transfer 1.0 nanomole of GlcNAc (N-Acetyl-D-glucosamine) from UDP-GlcNAc (Uridine diphosphate N-acetylglucosamine) to Galβ1→4Glcβ-O-pNP (4-Nitrophenyl β -D-lactopyranoside) per min at pH 7.5 at 25 °C.
Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.
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Beta-(1,3)-N-Acetylglucosaminyltransferase Size: 100 mU
Introduction: B3GlcNAcT is a β-1,3-N-acetylglucosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans. It can function also as a Beta-(1,3)-N-Acetylgalactosaminyltransferase
Activity: Overnight incubation with 4 µL of this enzyme will produce 1 mg of product. If higher amount of product is needed the enzyme will continuously add GlcNAc for at least one week. Reaction conditions are: Donor: UDP-GLcNAc, substrate: pNP-Lactose, product: pNP-Lactose-GlcNAc, reaction temperature: 23 ºC , incubation time: 18 hours. The specific activity is 975.7 pmol/min/μg
Source: E. coli cells with a cloned bacterial B3GlcNAc Transferase gene.
Storage Buffer: The enzyme is provided as lyophilized powder solution containing Tris buffer and NaCl
Milliunit Definition: One milliUnit (mU) is defined as the amount of enzyme that catalyzes the formation of 1 nanomol of 4-Nitrophenyl β -D-lactopyranoside-GlcNAc from UDP-GlcNAc (Uridine diphosphate N-acetylglucosamine) and Galβ1→4Glcβ-O-pNP (4-Nitrophenyl β -D-lactopyranoside) per min at 25 °C and at pH 7.5.
Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.
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IN STOCK
13C labeled asialo, mono-galactosylated biantennary complex N-glycan, with bisecting GlcNAc
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: bG1(6)
- Exact Mass: 1691.6422
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13C labeled bis-α-2,6-sialylated, galactosylated biantennary complex N-glycan, with bisecting GlcNAc
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: bG2S2
- Exact Mass: 2435.8859
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IN STOCK
13C labeled asialo, bis-galactosylated biantennary complex N-glycan, with bisecting GlcNAc
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: bG2
- Exact Mass: 1853.6950
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IN STOCK
13C labeled asialo, galactosylated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications – such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2, A2G2
- Exact Mass: 1648.6190
- Molecular Formula: C5413C8H104N4O46
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13C labeled di-sialylated, galactosylated biantennary
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2S2, A2G2S2, A2G2S2
- Exact Mass: 2068.7725
- Molecular Formula: C7013C8H128N6O57
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Introduction: CarboClip is a recombinant peptide-N-Glycosidase F (PNGase F), from Elizabethkingia sp, overexpressed in E. coli that contains a hexahistidine tag in its C-term and cleaves between the GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. CarboClip is provided glycerol-free as a lyophilized powder containing Tris-HCl, NaCl2, and Na2EDTA. Activity: After reconstitution in 150 μL of UltraPure H2O, 1 μl of CarboClip can deglycosylate > 95% of ribonuclease B (1 mg) in less than 20 minutes. Activity is calculated on a 12 % SDS-PAGE and percent of deglycosylation using densitometry. Storage: Lyophilized powder: -80 °C and -20 °C >1 year. At room temperature 10 days. Reconstituted protein should be stored at 4 °C and is stable for 2-3 months. Glycosylation of Membrane Proteins: Removal of N-Linked Glycans from membrane protein samples using this enzyme has been described by Contessotto et al. Matrix Biol. 2019 (See applications section below)
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The mAbsolute Human Serum IgG kit contains pauci-mannose, truncated, biantennary and bisected isotope-labeled standards including α-2,6-sialylation. The mixture of standards has been designed for the typical Fc and Fab N-glycosylation profile of IgG 1 type antibodies from human serum.
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13C labeled asialo, galactosylated triantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: FG3, FA3G(4)3, F(6)A3G(4)3
- Exact Mass: 2161.8158
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13C labeled asialo, agalacto, biantennary complex N-glycan, with bisecting GlcNAc
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G0B, A2B
- Exact Mass: 1529.5994
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13C labeled asialo, tri-galactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names:A4G3
- Exact Mass: 2215.9699
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13C labeled asialo, galactosylated tetraantennary complex N-glycan, with one unit of N-acetyllactosamine
- N-Glycan labeled with a single 13C atom per A4 structure GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names: A4G4 LacNac
- Exact Mass: 2743.0122
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13C labeled asialo, tetragalactosylated tetraantennary complex N-glycan
– N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
– Alternative glycan names: A4G4
– Exact Mass: 2378.1105 -
IN STOCK
13C labeled asialo, tri-galactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names:A4G3
- Exact Mass: 2215.9699
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13C labeled asialo, mono-galactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names: A4G1
- Exact Mass: 1891.6887
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IN STOCK
13C labeled asialo, mono-galactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names: A4G1
- Exact Mass: 1891.6887
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IN STOCK
13C labeled asialo, mono-galactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names: A4G1
- Exact Mass: 1891.6887
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IN STOCK
13C labeled asialo, agalactosylated tetraantennary complex N-glycan
- N-Glycan labeled with a single 13C atom per GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names:A4
- Exact Mass:1729.5481
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13C labeled di-sialylated, galactosylated biantennary, core-sub with fucose, bisecting GlcNAc
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: FA2BG2S2, F(6)A2BG2S2
- Exact Mass: 2581.9538
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IN STOCK
13C labeled di-sialylated, galactosylated biantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2FS2, FA2G2S2, F(6)A2G2S2
- Exact Mass: 2376.8678
- Molecular Formula: C8213C8H148N6O66
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IN STOCK
13C labeled di-sialylated, galactosylated biantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2FS2, FA2G2S2, F(6)A2G2S2
- Exact Mass: 2376.8678
- Molecular Formula: C8213C8H148N6O66
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IN STOCK
13C labeled di-sialylated, galactosylated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: A2, G2S2, A2G2S2
- Exact Mass: 2068.7725
- Molecular Formula: C7013C8H128N6O57
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13C labeled mono-sialylated, mono-galactosylated biantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G1FS2, FA2G1S2, F(6)A2G1S2
- Exact Mass: 1923.6607
- Molecular Formula: C6513C8H121N5O53
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13C labeled mono-sialylated, mono-galactosylated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G1S1, A2G1S1
- Exact Mass: 1777.6616
- Molecular Formula: C6513C8H121N5O54
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13C labeled mono-sialylated, galactosylated biantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: A1F, G2FS1, FA2G2S1, F(6)A2G2S1, G2A1(6)F
- Exact Mass: 2085.7723
- Molecular Formula: C7113C8H131N5O58
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Introduction:
- 13C labeled mono-sialylated, galactosylated biantennary, core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: A1F, G2FS1, FA2G2S1, F(6)A2G2S1, G2A1(6)F
- Exact Mass: 2085.7723
- Molecular Formula: C7113C8H131N5O58
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IN STOCK
13C labeled mono-sialylated, galactoyslated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2S1, S2G2S1, G2A1(6)
- Exact Mass: 1939.7144
- Molecular Formula: C6513C8H121N5O54
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Introduction:
13C labeled mono-sialylated, galactoyslated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2S1, S2G2S1, G2A1(3)
- Exact Mass: 1939.7144
- Molecular Formula: C6513C8H121N5O54
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Out of stock
IN STOCK 13C labeled mono-sialylated, galactoyslated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2S1, S2G2S1, G2A1(3)
- Exact Mass: 1939.7144
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13C labeled asialo, galactosylated biantennary core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G2F, FA2G2, F(6)A2G2
- Exact Mass: 1794.6769
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13C labeled asialo, monogalactosylated biantennary core substituted with fucose
- N-Glycan labeled with 2 13C atms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G1F, FA2G1, F(6)A2G1
- Exact Mass: 1632.6241
- Molecular Formula: C5413C8H104N4O45
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IN STOCK
13C labeled asialo, monogalactosylated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G1, A2G1.
- Exact Mass: 1486.5662
- Molecular Formula: C4813C8H94N4O41
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IN STOCK
13C labeled asialo, monogalactosylated biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G1, A2G1
- Exact Mass: 1486.5662
- Molecular Formula: C4813C8H94N4O41
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13C labeled asialo, agalacto biantennary core-substituted with fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G0F, FA2, F(6)A2
- Exact Mass: 1470.5713
- Molecular Formula: C4813C8H94N4O40
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13C labeled asialo, agalacto biantennary complex N-glycan
- N-Glycan labeled with 2 13C atoms on each GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G0, A2
- Exact Mass: 1324.5134
- Molecular Formula: C4213C8H84N4O36
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13C labeled Oligomannose 5
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G0-N, A1, MGn(6)
- Exact Mass: : 1119.3852
- Molecular Formula: C3613C6H71N3O31
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13C labeled asialo, agalacto truncated biantennary with core fucose
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: G0F-N, F(6)A1, MGn(3)F
- Exact Mass: 1265.4852
- Molecular Formula: C4213C6H81N3O35
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13C labeled Oligomannose 3
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: Man3, M3
- Exact Mass: 914,7809
- Molecular Formula: C3013C4H58N2O26
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13C labeled asialo, agalacto triantennary, core-substituted with fucose.
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: FA3, F(6)A3
- Exact Mass: 1675.6574
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13C labeled asialo, agalacto triantennary.
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: A3.
- Exact Mass: 1529.5994.
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The mAbsolute Complete kit also contains isotope-labeled standards for the three most frequently found α-2,3-sialylated glycans. Although they naturally occur in low amounts at the Fc part of an antibody, they can strongly influence effector function.
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The mAbsolute Neutral kit library of bi-antennary, heavy isotope-labeled N-glycan standards has been designed for the typical, CHO cell-produced, Fc N-glycosylation profile of monoclonal antibodies.
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13C labeled asialo, galactosylated tetraantennary core-substituted with fucose, with one unit of N-acetyllactosamine
- N-Glycan labeled with a sinlgle 13C atom per A4 structure GlcNAc, for use as a quantitative standard in applications such as MALDI-TOF, ESI,LC-MS…
- Alternative glycan names:
- Exact Mass: 2889.0702
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13C labeled Oligomannose 5
- N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
- Alternative glycan names: Man5, M5
- Exact Mass: 1238.4468
- Molecular Formula: C4213C4H78N2O36
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N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase Size: 4 mU
Introduction: B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose, Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and N-Acetyllactosamine (LacNAc) Type 1 O-glycans.
B3Gal Transferase can be used for synthesis of the precursor of H type 1 antigen of ABO histo-blood group. The H type 1 antigen in itself is the precursor of the following type 1 antigens: antigens A, antigen B, Lewis a (Le a ), Lewis b (Le b ) and Sialyl Lewis a (sLe a).
Source: E. coli cells with a cloned bacterial B3GalT gene.
Storage Buffer: The enzyme is provided in solution containing Tris buffer, NaCl and 10% glycerol
Activity: The activity per µL is 40 µUnits/µL or 248 pmol/min/μL
Milliunit Definition: One milliunit will transfer 1.0 nanomole of galactose from UDP-galactose to 4-Nitrophenyl N-acetyl-β-D-glucosaminide per min at pH 7.5 at 37 °C.
Storage: Store at -20 °C >1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.