Lectin Arrays

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Our lectin array service provides a high throughput platform for the rapid characterization of glycosylation in recombinant proteins, serum proteins, exosomes or ECM proteins

The technology utilizes a panel of nearly 50 different lectins as probes for testing the presence of a large number of biologically relevant glycan binding motifs and structural elements simultaneously on N- and O-glycans as well as glycolipids without requiring previous deglycosylation. We have over 15 years experience in lectin microarray development and analysis covering recombinant glycoproteins, serum glycoproteins, synthetic neoglycoproteins, cells, exosomes or extracellular matrix (ECM) proteins.

Over 48 highly purified lectins isolated from natural sources with well established glycan binding specificities are printed in 5 replicates alongside control samples (printing buffer containing BSA). We print our lectin microarrays on premium Schott Nexterion 3D hydrogel glass slides with ultralow background and excellent protein stabilisation properties.

Please inquire for custom array development if you have larger sample numbers or are interested in a specific lectin panel.

Subarray design example: current lectin distribution can differ from shown slide design, please inquire.

Method Summary

Glycoanalysis is performed in triplicate.

Glycoproteins samples are fluoresecently labeled, incubated on the lectin array and the interactions quantified by fluorescence detection. semiquantitative binding data is provided as histograms showing the corresponding lectin interaction profiles for each sample. Raw data (xlsx format) and a fluorescence image of the incubated lectin microarrays is also provided.

TypeLectinSpecificityMonosaccharide
AAAAnguilla anguilla lectinα-L-fucoseα-L-fucose
AALAleuria AurantiaFucFuc
ABLAgaricus bisporusGal(β1-3)GalNAc, T antigenGal
ACAAmarantus CaudatusT antigen, (Galβ1-3GalNAcα-Thr/Ser)Gal
AHPArachis hypogaea AHAGal(β1-3)GalNAc, T AntigenGal
AIA/JACArtocarpus integrifolia (Jacalin)T antigenGalNAc
AOLAspergillus orzyaeFucoseFuc
ASAAllium sativum lectinα1,3-mannoseMan
BPLBauhinia PurpureaGalβ-1,3 or β1,4-GalNAcGal
BS-I/GS-IGriffonia simplicifolia Iα-GalGal
BS-II/GS-IIGriffonia simplicifolia IITerminal GlcNAcGlcNAc
CALCicer arietinumFetuin, Lac, IgMLac
CFLCodium fragileGalNAcGalNAc
ConAConcanavalin Aα-Man, α-GlcMan
DBL/DBADolichos biflorusα-GalNAcGalNAc
DSLDatura stramonium(GlcNAc)2, LacNAcGalNAc
ECAErythrina Cristagalli AGal, GalNacGalNAc
EEAEuonymus EuropaeusLac, blood groups B and HGal
GNAGalanthus Nivalisterminal α1,-3ManMan
HALHelix aspersaGalNAcGalNAc
HMAHomarus americanusLAg1: NeuNAc. LAg2: GalNAcSialic acid, GalNAc
HPLHelix pomatiaα-GalNAc terminalGalNAc
LBAPhaseolus lunatusGalNAc-α1,3[Fuc-α1,2]GalGalNac
LELLycopersicon esculentum(GlcNAc)3GlcNAc
LTLLotus tetragonolobusTerminal α-Fuc, LexFuc
MAL-IMaackia amurensis Lectin Iα2,3-SialLacNacSial
MAL-IIMaackia amurensis Lectin IILacNAcGal
MOAMarasmium oreades agglutininGal-α1,3-Gal and Gal-α1,3-Gal-β1,4-GlcNAcα-Gal
MPAMaclura PomiferaT antigen, α-GalNAcGal
NPLNarcissus PseudonarcissusTerminal and internal ManMan
PALPseudomonas aeruginosa PA-IGalGal
PHA E+LPhaseolus Vulgaris AgglutiningoligomersLac
PNAPeanut agglutininT antigen, Gal(β-1,3) GalNacGal
PSAPisum sativumFucα-1,6-GlcNAc and α-ManFuc
PT-IPsophocarpus tetragonolobus Iα-GalNAcGalNAc
PT-IIPsophocarpus tetragonolobus IIα-1,2-fucosylated LacNAcGal
PWAPhytolacca americana(GlcNAc)3GlcNAc
RCA120Ricinus Communis Agglutinin,β-Gal, Lac, LacNAcGal
SBASoybean agglutininα-Gal-GalNAcGalNAc
SJASophora japonicaGalNAcGalNAc
SNASambucus nigraα-2,6 sialic acid on
LacNAc
Sialic, Lac
SSASalvia sclarea lectinTerminal GalNAc linked to serineGalNAc
STLSolanum tuberosum(GlcNAc)3, LacNAcGlcNAc
UEA-IUlex Europaea Agglα-1,3-Fuc. L-fucoseFuc
VFAVicia faba lectinα-Man, Glc, GlcNAcMannose
VVLVicia villosa B4GalNAcGalNAc
WFLWisteria floribundaGalNAcGalNAc
WGATriticum Vulgaris(GlcNAc)n, sialic acidGlcNAc

Promotional offer

Our current offer for lectin array analysis :

  • Optional sample preparation and protein isolation step (not included)
  • Sample labeling with fluorescent dye and sample cleanup
  • Optimization of sample concentration and incubation conditions on test slide
  • Lectin binding analysis in triplicate (4 samples/slide)
  • Microarray quantification, data analysis and data visualisation as histograms
  • Report including histograms, data file and discussion

Cost: Project for 4 samples measured in triplicate (2 slides are used for optimization and analysis) 2700,00€, additional samples (4 samples /slide, triplicate analysis) 1350,00€/slide

Please inquire for customized quotations for larger sample numbers or if you do not require triplicate measurements.