AplicationsUser's guideAplications B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose, Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and N-Acetyllactosamine (LacNAc) Type 1 Oligomers. User's guide
AplicationsUser's guideAplications B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose, Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and N-Acetyllactosamine (LacNAc) Type 1 Oligomers. User's guide
AplicationsUser's guideDocumentationMALDI-TOF PNGase FCitationsAplications Removal of N-Linked Glycans from Glycoproteins, Microwave deglycosylation, Proteomics, Glycomics… User's guide Booklet CarboClip 500 mU Booklet CarboClip 2500 mU Denaturing deglycosylation of 100 µg of glycoproteins and analysis Add 100 µg of glycoprotein and fill with UltraPure H2O (milliQ, NanoPure, etc) until 70 µl. Add 10 µl of Denaturing Buffer [...]