Enzymes
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AplicationsUser's guideAplications B3GalT is a recombinant N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase. The enzyme transfers galactose from UDP-galactose to the N-acetylglucosamine or N-acetylgalactosamine termini of acceptor molecules such as GalNAc, GlcNAc, Lacto-N-triose, Globotetrose, Isoglobotetrose and GalNAcβ1,3Galβ1,4Glc. Besides, B3GalT has been used to produce the Human Milk Oligosaccharide (HMO) lacto‐N‐tetraose (LNT) and N-Acetyllactosamine (LacNAc) Type 1 Oligomers. User's guide B3GalT [...] -
AplicationsUser's guideAplications B3GlcNAcT is a β-1,3-N-acetylglucosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans. User's guide -
AplicationsUser's guideAplications B3GlcNAcT is a β-1,3-N-acetylglucosaminyltransferase that transfers glucosamine from UDP-glucosamine to the Galactosyl termini of acceptor molecules such as Lactose (Galβ1→4Glc), Lactosamine (Galβ1→4GlcNAc), O-glycans (Galβ1→3GalNAc), Lacto-N-biose (Galβ1→3GlcNAc), Galβ1→6GlcNAc, Galβ1→4Xylβ-o-Bn or Biantennary, Triantennary and Tetraantennary N-Glycans. User's guide -
Deglycosylation ProtocolCitationsApplicationsUser GuideGlycan Mass SpectrometryDeglycosylation Protocol PNGase F Deglycosylation Protocol Denaturing deglycosylation of 100 µg of glycoproteins and analysis Add 100 µg of glycoprotein and fill with UltraPure H2O (milliQ, NanoPure, etc) until 70 µl. Add 10 µl of Denaturing Buffer (5% SDS, 400 mM DTT). Incubate at 100°C for 10 minutes. Add 10 µl of [...]