Lectin Microarray Our lectin array system provides a high throughput platform for rapid characterization of glycosylation in proteins, antibodies, hormones, and other glycoconjugates. The technology utilizes a panel of up 48 different lectins that specifically bind to different N and O- glycans epitopes including :
Blood groups B and H
Lewis X antigen
Complex branched glycans
The panel includes 6 replicates of each immobilized lectin along with immobilized control samples (printing buffer containing BSA) Method Summary Analyses are performed in triplicate. The glycoproteins samples are labeled with a cy-3/cy-5 dyes, incubated with the lectin array and the interactions quantified by fluorescence detection. Data is provided as histograms showing the corresponding lectin interaction profiles with each sample. Raw data (xlsx format) and a fluorescence image of the incubated lectin microarrays is also provided Pricing Prices are based in the number of chips used.
AplicationsUser's guideDocumentationMALDI-TOF PNGase FCitationsAplications Removal of N-Linked Glycans from Glycoproteins, Microwave deglycosylation, Proteomics, Glycomics… User's guide Booklet CarboClip 500 mU Booklet CarboClip 2500 mU Denaturing deglycosylation of 100 µg of glycoproteins and analysis Add 100 µg of glycoprotein and fill with UltraPure H2O (milliQ, NanoPure, etc) until 70 µl. Add 10 µl of Denaturing Buffer [...]