Absolute Glycan Quantification by MS using Stable Isotope Labeled Glycan Standards
CarboQuant standards are patented synthetic 13C-labeled N-glycans, produced entirely in our laboratory for use as internal standards in absolute glycan quantification by mass spectrometry. They are available as single standards or in kit form including a quantification software and all reagents and consumables required for glycan analysis.
CarboQuant standards
- >95% pure
- Fully characterized by MS and NMR
- Show up to 8 Dalton mass gain. No peak overlap
- All N-acetylglucosamine are labelled
- Quantified internal standards (qNMR )
- Broad coverage of N-glycan structures
Applications
- Use of internal glycan standards to improve your glycan analysis workflow
- Improve your method robustness with internal glycan standards
- Quantify individual glycans in biofluids like any other metabolite
- Use MALDI-TOF MS to generate quantitative glycan profiles in biomarker studies and biopharmaceutical research with very fast sample preparation and acquisition
mAbsolute Kits
- Glycan Absolute Quantification by Mass Spec. using Stable Isotope Labeled Glycan Standards
Each kit contains
- Quantified mixture of 8-11 13C-standards covering all major glycans present
- Proprietary peptide N-glycosidase F (PNGase F)
- Sialic acid stabilization procedure
- Proprietary Quantification Software
in Glycobiology
- Rapid Absolute glycan quantification
- Measure of glycan recovery for in sample preparation
- Internal standards for the quantitative glycan profiling by MALDI-Tof MS employing mAbsolute kits
Biopharmaceutical development
- Rapid high-throughput glycan analysis in clone selection and process development
- Quality control
- Biosimilar optimisation
Clinical Chemistry Biomarkers
- Measure glycan levels in serum samples with picomole precision
- Establish cut-off values for glycan biomarkers
- Quantify aberrant glycosylation in cancer samples
- Internal performance calibrants for method transfer and multiple laboratory studies
References
1. Synthesis and use of isotopically-labelled glycans. EP 2981541 A1
2. Anal Chem. 2015 Nov 17;87(22):11460-7
