Technology

Absolute Glycan Quantification by MS using Stable Isotope Labeled Glycan Standards

CarboQuant standards are patented synthetic 13C-labeled N-glycans, produced entirely in our laboratory for use as internal standards in absolute glycan quantification by mass spectrometry. They are available as single standards or in kit form including a quantification software and all reagents and consumables required for glycan analysis.

CarboQuant standards

  • >95% pure
  • fully  characterized by MS and NMR
  • show up to 8 Dalton mass gain. No peak overlap
  • All N-acetylglucosamine are labelled
  • Quantified internal standards (qNMR )
  • broad coverage of N-glycan structures

Applications

  • Use of internal glycan standards to improve your glycan analysis workflow
  • Improve your method robustness with internal glycan standards
  • Quantify individual glycans in biofluids like any other metabolite
  • Use MALDI-TOF MS to generate quantitative glycan profiles in biomarker studies and biopharmaceutical research with very fast sample preparation and acquisition

mAbsolute Kits

  • Glycan Absolute Quantification by Mass Spec. using Stable Isotope Labeled Glycan Standards

Each kit contains

  • Quantified mixture of 8-11 13C-standards covering all major glycans present
  • Proprietary peptide N-glycosidase F (PNGase F)
  • Sialic acid stabilization procedure
  • Proprietary Quantification Software

in Glycobiology

  • Rapid Absolute glycan quantification
  • Measure of glycan recovery for in sample preparation
  • Internal standards for the quantitative glycan profiling by MALDI-Tof MS employing mAbsolute kits

Biopharmaceutical development

  • Rapid high-throughput glycan analysis in clone selection and process development
  • Quality control
  • Biosimilar optimisation

Clinical Chemistry Biomarkers

  • Measure glycan levels in serum samples with picomole precision
  • Establish cut-off values for glycan biomarkers
  • Quantify aberrant glycosylation in cancer samples
  • Internal performance calibrants for method transfer and multiple laboratory studies

References

1. Synthesis and use of isotopically-labelled glycans. EP 2981541 A1

2. Anal Chem. 2015 Nov 17;87(22):11460-7

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